Angelo Coast Range Reserve

12 Aug, 06 AM - 23 Aug, 10 AM

This project will explore factors influencing the nature of the interactions between biofilm-associated algae and bacteria in the streams of the Angelo Coast Range Reserve. Nutrient and/or biocide-diffusing substrates will be deployed in streams covering a wide range of catchment size. Nutrients and biocides will be dissolved in agar and placed inside of semi-permeable substrates to strictly constrain the spatial extent of their influence. Selective biocides developed for pond and aquarium use will be used to exclude bacteria or algae from experimental substrates so the performance of the other interactor can be measured alone. This will help to determine how landscape position and stream size affect the influence of resource limitation on the nature of bacterial-algal interactions. At the end of each 4-week deployment substrates will be harvested and analyzed for chlorophyll A, ash-free dry mass, bacterial and algal abundance, and DNA restriction fragment length polymorphisms (a measure of diversity). Field sites for this project may include the following streams: Ten Mile, South Fork Eel, Barnwell, McKinley, Fox, Elder, Misery, Paralyze, and Jack of Hearts. Specific locations on these streams will be determined based on the activities of other researchers working in these areas. Every effort will be made to avoid any damage to sensitive areas or interference with other research. Lab facilities will be used for preliminary processing and preservation of samples.

Visit #8067 @Angelo Coast Range Reserve

Approved

Under Project # 5579 | Research

Bacterial-Algal Interactions in Epilithic Biofilms

graduate_student - University of California, Berkeley

Reservation Members(s)

Maria Goodrich Aug 12 - 23, 2005 (12 days)

Reserve Resources(s) | Create Invoice

Computer lab 1 Aug 12 - 23, 2005
Fox Creek Lodge 1 Aug 12 - 23, 2005
Lab 1 Aug 12 - 23, 2005
Wilderness Lodge 1 Aug 12 - 23, 2005