Landels-Hill Big Creek Reserve

14 Aug, 08 AM - 15 Aug, 05 AM

Previous work on allozyme markers has shown that natural selection may affect the observed polymorphisms at the Lactate Dehydrogenase-A (LDH-A) locus in the high cockscomb (A. purpurescens) along the California coast. I am proposing to shed further light on this matter by examining DNA polymorphism at the LDH-A gene, and comparing this to variation at six other genetic markers. Collections I am planning on collecting 10-20 individuals of A. purpurescens from six sites along the California coast. Individuals will be collected by hand under exposed rocks and boulders at low tides. Laboratory Work DNA will be isolated using established laboratory protocols. Portions of the LDH-A, Aldolase-C, Creatine Kinase, Myostatin, Beta-tubulin, Growth hormone, and Cytochrome B genes will be amplified with the polymerase chain reaction (PCR). PCR products will be sequenced on and ABI 377 automated sequencer. Analysis General polymorphism statistics (number of segregating sites, nucleotide diversity, and Tajima?s D) will be estimated for all genes sequenced. Population differentiation will be estimated using standard and coalescent-based methods. Measures of polymorphism and population differentiation will be compared for the LDH-A gene and all other genes sampled. It is expected that if selection has acted upon the LDH-A gene, it will deviate significantly in these measures from the other sampled genes.

Visit #5752 @Landels-Hill Big Creek Reserve

Approved

Under Project # 4490 | Research

A test for selective signatures on the Lactate Dehydrogenase-A gene in Anoplarchus purpurescens

faculty - University of California, Merced

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Andres Aguilar Aug 14 - 15, 2004 (2 days)

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