Box Springs Reserve

29 May, 11 AM - 30 May, 03 PM

Genetic sampling of Peromyscus rodents to understand genetic mechanisms of dehydration tolerance. we aim to live-trap and sample ear-clips from deer mice (Peromyscus eremicus and crinitus) for sequencing back at the MacManes Lab at the University of New Hampshire. Standard sherman traps will be set in the evening and collected in the morning to limit heat exposure. Trapped animals will be partially anesthetized using isofluorane, standard mammalogical measurements (weight [g], total length, tail length, length of hindfoot and length of ear, reproductive status), will be collected in addition to recording metabolic activity via electrolytic screening of blood draws from the facial vein (punctured near the bend in the jaw using a small lancet, and collected via capillary tube). Ear clips will be taken with scissors and blood will be drawn from the facial vein (near the distal bend in the lower jaw) using a small lancet to test individual electrolyte levels. After data-collection all but 2 male and 2 female deer mice will be released - these 4 samples will have full tissues subsampled into RNAlater for gene expression analyses.

Visit #67411 @Box Springs Reserve

Approved

Under Project # 44953 | Research

Peromyscus dehydration genomics

research_scientist - University of New Hampshire

Reservation Members(s)

Jocelyn Colella May 29 - 30, 2020 (2 days)
Group of 1 Graduate Student May 29 - 30, 2020 (2 days)

Reserve Resources(s) | Create Invoice

Day use only 2 May 29 - 30, 2020